Abbott CELL-DYN 3000 - Measurement Specifications; Measurement Channels; WBC and Differential; Rbcs and Plts

CELL-DYN 3000 System Operator’s Manual 4-9
9140240E — May 1995
Chapter 4 System Specifications
Measurement Specifications — CELL-DYN 3000SL and CS
Measurement Channels
Impedance channel for both RBC and PLT
Laser Optics for WBC and Differential
Hemoglobin Absorbance
WBC and Differential
Method Laser light scatter
Light Source Vertically polarized 5–10 mW helium neon laser
Wavelength 632.8 nm
Dilution 1:51 of blood in sheath reagent
Data Collection Four angles measured:
0
°, 10°, 90° and 90° depolarized.
Data collected in 256 channels for each angle of
light scatter.
RBCs and PLTs
Method Aperture Impedance
Aperture Size 60 µm (diameter) x 72 µm (length)
Dilution 1:12,501 of blood in diluent
Data Collection 256 channels for RBCs,
each RBC channel = 1 fL
256 channels for PLTs,
each PLT channel = 0.137 fL
HGB
Method Modified hemiglobincyanide
Light Source LED
Wavelength 540 nm
Dilution 1:251 of blood in diluent and
Hemoglobin Lyse
Data Collection Average of 5 absorbance readings for the blank,
average of 5 absorbance readings for the sample
dilution
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