3-8 CELL-DYN 3000 System Operator’s Manual
9140240E — May 1995
Principles of Operation Chapter 3
Figure 3.2: WBC Light Scatter
Sheath Reagent
The Sheath reagent is an integral part of the WBC analysis. White blood
cells diluted in the Sheath reagent maintain cellular integrity close to
their native state. The structure of the basophils changes slightly due to
the water soluble nature of the basophilic granules.
The RBCs are also altered by the Sheath reagent. The RBCs are ruptured
by hypo-osmotic shock. The RBC ghosts have the same refractive index
as the Sheath reagent, thereby rendering them invisible to the laser.
Detection with the Optical Bench
The optical bench assembly contains the components that make up the
flow cytometer. It is depicted in Figure 3.3. The main purpose of the
optical bench is to detect the light scattered by the cells as they pass
through the flow cell. The detection process is discussed in this section.
Focused
Laser Beam
Various Angles
of Scattered Light
90° Scatter
90°D Scatter
0° Scatter
10° Scatter
To Next Page
Loading...
