3-16 CELL-DYN 3000 System Operator’s Manual
9140240E — May 1995
Principles of Operation Chapter 3
Once the WBC count is determined, the absolute number of cells in each
subpopulation is calculated by multiplying that WBC count by the
percentage. The results are expressed as follows:
WBC # x 10
3
/µL
NEU # x 10
3
/µL and %
LYM # x 10
3
/µL and %
MONO# x 10
3
/µL and %
EOS # x 10
3
/µL and %
BASO # x 10
3
/µL and %
The WBC subpopulations are further identified by the following colors:
Neutrophils —yellow
Lymphocytes —blue
Monocytes —magenta
Eosinophils —green
Basophils —white
The WBC scatter information is usually displayed in two scatterplots as
shown in Figure 3.8:
SIZE/COMPLEXITY: The size (0
° scatter) information is plotted
on the Y axis and the complexity (10
°
scatter) information is plotted on the X
axis.
DEPOL/ORTHOGONAL: The granularity (Depol 90
°D scatter)
information is plotted on the Y axis and the
lobularity (Orthogonal 90
° scatter)
information is plotted on the
X axis.
WBC Flagging
Refer to the “Operational Messages and Data Flagging” section of this
chapter for WBC flagging information.
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