5-68 CELL-DYN 3000 System Operator’s Manual
9140240E — May 1995
Operating Instructions Chapter 5
Sample Collection
All samples should be collected using proper technique and following
the tube manufacturer’s recommendations.
NOTE: For additional information on collecting venous and
capillary samples, refer to NCCLS Standards, H3-A3
1
and H4-
A3
2
.
The recommended tube for the Sample Loader is a 13 x 75 mm tube with
a hemoguard closure that draws 3 mL of blood. The sample volume in
this tube ensures proper mixing by the Sample Loader.
A minimum of 250 µL should be collected for micro-collection samples.
This ensures an adequate amount of blood for the Open Mode (170 µL)
aspiration.
Interfering Substances
The CELL-DYN 3000 has been designed to detect and flag samples that
contain interfering substances. The following list indicates the
substances that may interfere with the listed parameters.
WBC: NRBCs, lytic-resistant RBCs, PLT clumps,
cryoglobulin and cryofibrinogen, fragile WBCs
RBC: Elevated WBC count, increased numbers of giant
PLTs, autoagglutination, in vitro hemolysis
HGB: Elevated WBC count, increased plasma substances
(triglycerides, bilirubin, in vivo hemolysis), lytic-
resistant RBCs
MCV: Elevated WBC count, hyperglycemia, in vitro
hemolysis, increased numbers of giant PLTs
PLT: WBC fragments, in vitro hemolysis, microcytic RBCs,
cryoglobulins, PLT clumping, increased numbers of
giant PLTs
For a detailed description of the flags that are generated, refer to the
Operational Messages and Data Flagging section of Chapter 3, Principles
of Operation.
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