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into a cartridge. Help graphics on the instrument show transfer of Cal Ver material to the cartridge via

syringe with blunt p needle.

7. Immediately close the cartridge and insert it into an instrument – it is important to prevent exposing

the soluon to room air since this will alter the results.

Transferring the Cal Ver soluon: syringe with blunt p needle

Plain syringes (1cc or 3cc sterile syringes with blunt p needles) are recommended to transfer aqueous

Cal Ver soluons from the ampule to the cartridge.

To use a syringe:

1. Place the end of the blunt p needle into the boom of the ampule.

2. Slowly remove approximately 1cc of soluon from the ampule into the syringe.

• If air is trapped between the leading edge of the soluon and the plunger, do not invert the

syringe to expel it; this will not aect soluon near the p of the syringe.

• If air bubbles are connually drawn into the syringe, or if a bubble is trapped near the p of the

syringe, discard the ampule and syringe.

3. Remove syringe from the ampule and expel one or two drops from the syringe.

4. Place needle into the sample cartridge and ll the cartridge to the ll mark.

5. Close cartridge and begin tesng immediately.

Transferring the Cal Ver soluon: capillary tube

Plain capillary tubes are recommended to transfer aqueous control soluon from the ampule to the

cartridge. Capillary tubes with sucient ll capacity are required. For example, when lling a cartridge

that requires 95 µl, use a 150 µl capillary tube.

To use a capillary tube:

1. Place a clean, dry nger over one end of the tube and insert open end of the tube into the boom of

the ampule.

2. When the open end of the tube touches the boom of the ampule, p the ampule slightly. Remove

nger from the other end to allow lling by capillary acon. Completely ll the tube with the Cal Ver

soluon.

3. Place a nger over the open end of the tube. Remove the tube from the ampule.

4. Place open end of the tube into the sample cartridge. Remove nger to allow the Cal Ver soluon to

ll the cartridge to the ll mark.

5. Close cartridge immediately aer lling.

6. Tesng should be started immediately.

Reacve ingredients for i-STAT Calibraon Vericaon materials

Analyte Cal Ver Level 1 Cal Ver Level 2 Cal Ver Level 3 Cal Ver Level 4 Cal Ver Level 5

Na (mmol/L) 108 127 141 169 187

K (mmol/L) 2.3 3.1 4.0 6.8 8.5

Cl (mmol/L) 71 85 100 122 133

Glu (mmol/L) 1.8 2.5 7.3 17 35

Urea (mmol/L) 44.6 18 4 2.7 1.8

i-STAT Alinity — System Operaons Manual Art: 745537-01 Rev. F Rev. Date: 02-Nov-2022

Abbott i-STAT Alinity User Manual

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