BD FACSAria - Page 261

Appendix B: Application Settings 261
a Adjust the FSC area scaling factor until the FSC-A signal matches the
FSC-H signal. See Figure B-5.
• Increase the area scaling factor if the APC-A signal is lower than
APC-H.
• Decrease the area scaling factor if the APC-A signal is higher than
APC-H.
• View the result of your change in the histograms and statistics views.
Figure B-5 FSC area scaling comparison
9 Adjust the blue laser area scaling until the FITC-A signal matches the FITC-
H signal, if needed.
10 Unload the FITC tube, then load the APC tube.
Incorrect FSC Area Scaling Correct FSC Area Scaling