44 1100 Series FD Reference Manual
2 First Steps with the Fluorescence Detector
Tabl e 7 Conditions for Figure 13 and Figure 14 on page 45
Column Vydac, 2.1 x 200 mm, PNA, 5 µm
Mobile phase A = water; B = acetonitrile (50:50)
Gradient 3 minutes, 60%
14 minutes, 90%
22 minutes, 100%
Flow rate 0.4 ml/min
Column temperature 18
°C
Injection volume 5 µl
FLD settings PMT 12,
response time 4 s,
step size 5 nm
Figure 13 Optimization of the time-program for the emission wavelength
This shows the
isofluorescence plot
of emission spectra
for 15 PNAs
(5 µg/ml) with a
fixed excitation
wavelengths
(260 nm).
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