First Steps with the Fluorescence Detector 2
1100 Series FD Reference Manual 45
The obtained data are combined to setup the time-table for the excitation
wavelength for best limit of detection and selectivity. The optimized switching
events for this example are summarized in Table 8.
This timetable gives the conditions for optimum detection based on the results
of two chromatographic runs.
Figure 14 Optimization of the time-program for the excitation wavelength
Tabl e 8 Timetable for the analysis of 15 polynuclear aromatic hydrocarbons
Time [min] Exitation Wavelength [nm] Emission Wavelength [nm]
0 260 350
8.2 260 420
19.0 260 500
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