Chapter 4 -Operational Procedures-
136
AS-410M Auto Slide Preparation System
4-12-1 Roughing parameters
Table 4-10 lists parameters available during the roughing. Select the microtome blade operation
mode and the surface angle adjustment method. Specify the sectioning thickness per cut and the
number of sections to define the roughing depth from the sample surface. Select whether to attach
thin sliced sections to the carrier tape during the roughing.
Table 4-10: Rough-cutting parameters
Screen to use
Parameter type Operation Parameters
Slicing
Parameter
Settings
Slicing parameter
Slicing parameter
file selection
1. Slicing speed
2. Knife pull angle (90° or 60°)
Sample Block
Positions
Sample tray and
position on it
1. Specify a tray and a sample
position on it.
2. Select all samples on the
specified tray.
3. Deselect all samples on the
specified tray.
Roughing
Condition
Settings
Slicing thickness
Slicing thickness
per cut
2 to 30
μ
m (increments of 1
μ
m)
(Recommended: 15
μ
m or less)
Number of sections
1 to 999 cuts
Humidifying
Sample
moistening
1. ON (moistening enabled)
2. OFF (moistening disabled)
Roughing
1. Attach sections to
carrier tape.
2. Abort roughing.
3. Pause roughing.
Check thin sliced
sections to
ensure they each
contain a tissue
slice.
After section attachment to the
carrier tape with Option 1 selected.
The System will automatically pause
itself
4-12-2 Outline of the operation and the preoperational setup
Refer to 4-13-3 “Outline of the roughing operation.”
As preparatory works before power-on, set sample blocks and the waste liquid tank in place (refer
to 3-4 and 3-8, respectively). Depending on the condition settings, the humidifying water bottle and
the carrier tape must be set in place (refer to 3-6 and 3-12, respectively). In roughing operation, slides,
slide collection boxes, and application medium are not necessary.
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