Chapter 4 -Operational Procedures-
126
AS-410M Auto Slide Preparation System
⑥ Section attachment to slides
Check the shape of sections attached to the slides.
1) Thin sliced sections are attached properly to the slides. No leftovers remain on the carrier
tape.
2) Sections attached to the slides are free from wrinkles or flaws.
Checking procedures
If faulty sectioning or adhesion to slides occurs when no change has been made to the
section thickness or the sample block preparation method, the cause may be one or more of
those given below.
When any change has been made to the sample block preparation method, select
appropriate parameters accordingly.
① Slicing parameters
Changes to the sectioning parameters affect the sectioning and put-on movement. Check and
ensure that the parameter settings are the same as the last time the System worked normally.
To check the currently selected basic parameters, first select PARAMETER SETTING from the
“MAIN” screen. Then, the file number and file name of the currently selected file will appear on the
upper section of the “Slicing Parameter Setting” screen.
② Scratched sectioning of the blade
If there are linear flaws in sections, it may be caused by a scratched sectioning blade or by a
paraffin buildup on the sectioning blade. Change the sectioning position. Alternatively, replace the
blade.
③ Application medium (drip water)
Application medium is dripped from the application medium nozzle onto slides that are pushed
out from the slide cassette after sample sectioning and section attachment to the carrier tape.
Check and ensure that medium is dripped accurately onto slides.
④ Lowering width, feeding speed, etc., of the carrier tape during section attachment
The lowering width, feeding speed, etc., of the carrier tape are factory-set sectioning parameters.
Contact us if the possible cause is the parameter settings of the carrier tape.
Carrier tape
Section
Slide glass
●
Adhesion of sections to slides may vary depending on the type of tissue
used, the tissue fixation method used, or the type of embedding paraffin
used. What follows describes actions to be taken for problems that may
arise from sample blocks prepared using conventional methods.
Put-on (mounting)
！ Note
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