APPLIED OPERATIONS/ Protocol processor
OPERATION INSTRUCTIONS
2-184
Page
One Point!
The protocol to perform the following operations can be assembled after setting the
observation conditions.
z Specifying the region for bleaching in the laser irradiation mode using REX mask
files.
In Track 4, select a REX mask file in [Mask] of [REX]. Then in the laser irradiation mode
specified by the REX mask file selected, images can be acquired. (When using the system
with AOTF.) To select a REX mask file, display the REX mask file in the image window in
advance.
<The setting example>
For details, see section 2-2-11, “Image Acquisition in the Laser Excitation Mode”.
z Setting bleaching region by use of rectangular image acquisition (RECT).
Wait 5
sec.
Set Zoom
power to x2
Set Zoom
power to x10
Get 5
images
（bleach）
Set Zoom
power to x2
Get one
image
Acquire
image
Save
image
Protocol
Track 1 Track 2
Track 3
Repeat 30 times
Set bleaching
portion
Wait 5
sec.
Acquire
image
Reference image acquisition:
Acquire an image with the Zoom power 2 x in Track 1 in XYT observation.
Photo-bleaching:
Expose laser to photo-bleach with 5 images of acquisition using ZoomIn mode in Track 2 in
XYT observation.
Recovery observation:
Acquire 3 images in XYT observation every 5 seconds with the zoom power 2 x, the field of
view is the same with the reference image, and the images are stored in Track 3.
<Example>
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