Olympus Fluoview FV1000 - Page 340

APPLIED OPERATIONS/Image Processing
OPERATION INSTRUCTIONS
2-256
Page
· Pearson’s Coefficient :
In case that the value to be acquired is defined as
r
,
∑∑
∑
−⋅−
−⋅−
=
ii
iiii
i
iiii
FFFF
FFFF
r
2
,2,2
2
,1,1
,2,2,1,1
)()(
)()(
However, legends used for image measurement are as follows:
i
F
,1
: Brightness of wavelength
1
λ
at i -th pixel,
i
F
,2
: Brightness of wavelength
2
λ
at
i -th pixel
∑
=
i
ii
F
N
F
,1,1
1
: Average brightness of wavelength
1
λ
,
∑
=
i
ii
F
N
F
,2,2
1
: Average
brightness of wavelength
2
λ
· Overlap :
In case that the value to be acquired is defined as
r
,
∑∑
∑
⋅
⋅
=
i
i
i
i
i
ii
FF
FF
r
2
,2
2
,1
,2,1
· Overlap Index #1 :
In case that the value to be acquired is defined as
1
k ,
∑
∑
⋅
=
i
i
i
ii
F
FF
k
2
,1
,2,1
1
· Overlap Index #2 :
In case that the value to be acquired is defined as
2
k ,
∑
∑
⋅
=
i
i
i
ii
F
FF
k
2
,2
,2,1
2
· Colocalization Index #1 :
In case that the value to be acquired is defined as
1
m ,
∑
∑
∈
=
i
i
Ai
i
F
F
m
,1
,1
1
However, Ai ∈ should be i -th pixel that belongs to aggregation A that is greater than
threshold value.
· Colocalization Index #2 :