Olympus Fluoview FV1000 - Page 76

Getting Started FLUOVIEW/Online Help
OPERATION INSTRUCTIONS
1-54
Page
One Point!
The formulas of resolution of X and Y are as follows;
FWHM : Resolution of X and Y
L : The length of a diagonal line of Square Pinhole
NA = nSINθ :NA of Objective lens
MG : The objective Magnification
N : The refractive index of a medium
Air : 1.0
Water : 1.3
Oil : 1.5
X = L/(1.22*3.8*λ*MG/NA)
The case where the variable x is as follows; 0 <= x < 0.5
FWHM = (0.5λ*XYPSF)/NA
XYPSF = -5.1459x
6
+ 1.0147*10x
5
- 7.1668x
4
+ 2.2804x
3
- 1.7696*10
-1
x
2
+ 1.9256*10
-02
x +
7.1915*10
-1
The case where the variable x is as follows; 0.5 <= x < 1.0
FWHM = (0.5λ*XYPSF)/NA
XYPSF = -3.05539x
6
+ 1.32031*10x
5
- 2.25074*10x
4
+ 1.89617*10x
3
- 7.89685x
2
+ 1.56587x
+ 6.20850*10
-1
The case where the variable x is as follows; 1.0 <= x < 1.7
FWHM = (0.5λ*XYPSF)/NA
XYPSF = -2.61786x
6
+ 2.14478*10x
5
- 7.18428*10x
4
+ 1.25767*10
2
x
3
- 1.21445*10
2
x
2
+
6.17335*10x - 1.21495*10
The case where the variable x is as follows; 1.7 <= x < 2.0
FWHM = (0.5λ*XYPSF)/NA
XYPSF = -0.0078x
3
+ 0.0422x
2
- 0.0689x + 1.0478
The case where the variable x is as follows; 2.0 <= x
FWHM = (0.5λ*XYPSF)/NA
XYPSF = 1.0164