Running Autosampling 109
4
7. Move the cursor to the Dispense Needle (%) field. Default is 100. The displayed
number is the needle dispense position in the sample vial.
Move the cursor to 25%, 50%, 75%, or 100%. To optimize mixing, select the
position which places the bottom of the needle in the middle of the solution in the
sample vial.
The Dispense Needle setting affects dispensing only. When the 717plus
Autosampler withdraws from the transfer vial or from the sample vial during mixing,
the needle position is at 100%.
8. Move the cursor to the Vial field. Enter the following parameters for up to five
transfer vials:
Parameter Description/Range
Vial Transfer vial (for withdrawal) number, 1 to 48 or 1 to 96.
Trans Vol Volume to withdraw from transfer vial: 0.0 to 150.0 µL or 0.0 to
1500.0 µL (dependent on sample loop installed).
Volume is limited to 60% of syringe size. A 250-µL syringe is
recommended for most applications.
# Mix Number of mix cycles, 0 to 9. A mix cycle withdraws a sample from
the sample vial and returns it to the same vial.
Mix Vol Volume of fluid withdrawn for mixing back to the sample vial:
0.0 to 150.0 µL or 0.0 to 1500.0 µL (depending on sample loop
installed).
Note that the volume is limited to 60% of the syringe size.
Purge Time 0.0 to 99.9 minutes.
Note that during an Auto Transfer purge, only the sample loop is
purged. The syringe is not purged and a compression check is not
performed.
Delay Time Time to delay before next transfer or injection, 0.0 to 99.9 minutes.
Delay and purge run simultaneously. For example, if you program a
3-minute Delay Time and a 1-minute Purge Time, the 717plus
Autosampler purges for 1 minute, then delays an additional
2 minutes.
If the purge time is programmed longer than the delay time, this
results in a delay that is as long as a purge.
STOP
Attention: Do not program a transfer volume greater
than the volume of the sample vial.
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