Table of Contents 15
Chapter 3
Startup and Configuration ................................................................ 55
3.1 Powering Up the Autosampler............................................... 55
3.2 Using the Front Panel............................................................ 56
3.2.1 Accessing Menus....................................................... 57
3.2.2 Entering Operating Parameters ................................. 58
3.3 Flushing the Autosampler ..................................................... 59
3.4 Calibrating the Seal Pak........................................................ 59
3.4.1 Purging the Autosampler ........................................... 59
3.4.2 Auto Zeroing the Transducer...................................... 60
3.4.3 Calibrating the Seal Pak ............................................ 62
3.5 Priming the Needle Wash Pump........................................... 64
3.6 Configuring the Autosampler ................................................ 65
3.6.1 Overview of Configuration ......................................... 65
3.6.2 Setting Parameters for LC System Operation............ 68
3.6.3 Entering Hardware Changes ..................................... 72
3.7 System Verification ............................................................... 74
3.8 Power Down and Storage ..................................................... 75
3.8.1 Removing Buffered Mobile Phase ............................. 75
3.8.2 Powering Down.......................................................... 75
3.8.3 Storage ...................................................................... 76
Chapter 4
Operating the Autosampler .............................................................. 77
4.1 Overview of Operation .......................................................... 77
4.2 Preparing Samples ............................................................... 79
4.2.1 Preparing Samples .................................................... 79