Table of Contents 16
4.2.2 Selecting Sample Vials.............................................. 81
4.3 Selecting a Needle Wash Solvent......................................... 82
4.4 Loading the Carousel............................................................ 84
4.5 Ensuring Reproducibility ....................................................... 86
4.5.1 Overview of Reproducibility ....................................... 86
4.5.2 Performing a Purge.................................................... 87
4.5.3 Performing a Compression Check............................. 89
4.6 Running Autosampling.......................................................... 91
4.6.1 Programming Auto Run ............................................. 92
4.6.2 Programming Step Functions .................................... 96
4.6.3 Using Auto Standards ............................................... 98
4.6.4 Using Auto Addition ................................................ 102
4.6.5 Using Auto Transfer ................................................. 106
4.6.6 Programming the Event Output ............................... 113
4.6.7 Starting and Stopping an Auto Run ......................... 114
4.6.8 Monitoring Auto Run Status..................................... 115
4.7 Making a Stat Run .............................................................. 116
4.8 Sampling Considerations .................................................... 119
4.8.1 Temperature Control ................................................ 119
4.8.2 Microbore/Semiprep Injections ................................ 120
4.8.3 Injecting Viscous Samples ...................................... 123
4.8.4 Purging During an Auto Run.................................... 124
4.8.5 Using Buffered Mobile Phase .................................. 125