Waters 717plus - Page 95

Running Autosampling 95
4
Step
1. Place the cursor on a blank line, then press Enter.
The 717plus Autosampler inserts a new line and copies the information, including
step function and option settings, from the previous line.
You can program up to 96 different steps. Each vial included in the step shares the
same number of injections, injection volume, run time, step functions, and options.
By changing steps and step parameters, you can run a different method using the
same set of vials.
Vial From/To
2. Enter a vial number in the From Vial and To Vial fields. Vial number defaults to the
values you entered on the previous line. Valid entries are 1 to 48 or 1 to 96,
depending on the type of carousel in use.
When injecting from a single vial, enter the same vial number in the From and To
fields. When injecting from a sequence of vials, enter the starting vial in the From
Vial field and the last vial in the To Vial field.
# Inj
3. Enter the number of injections to make from each vial. Valid entries are 1 to 99.
Inj Vol
4. Enter the injection volume. The maximum injection volume depends on the size of
the sample loop.
Run Time
5. Enter the run time in minutes. Valid entries are 0 to 999.9.
The run time clock starts when the sample enters the mobile phase stream. At the
same time, the autosampler closes the Inject Start contact. When the run time clock
expires, the autosampler closes the Inject Stop contact and begins another injection
sequence unless a Report Delay has been configured.
Note: You can delay the next injection in order to complete an integration printout.
Use the Report Delay or External Hold input signal (see Section 3.6.2,
Setting
Parameters for LC System Operation).
STOP
Attention: The vial injection sequence entered in the From/To field is
continuous. If a vial is missing, the autosampler will inject air into the LC
system.
Sample Loop Size Range (µL)
Standard 0 to 200
Auxiliary 0 to 2000