182 BD FACSAria User’s Guide
Table 6-1 Cytometer cleaning modes
Cleaning Mode Summary Frequency
Sample Line Backflush on
page 183
Flushes the sample line with
sheath fluid
After running samples with
adherent cells or dye
Clean Flow Cell on
page 184
Cleans the sample path and
the flow cell with a cleaning
solution
When indicated by
distorted scatter or high
CVs
Prime After Tank Refill on
page 185
Primes the fluid lines for the
designated fluid(s)
When a fluidics line is
unplugged to refill a tank
Long Clean on page 186 Decontaminates the internal
sheath path with bleach or
ethanol and rinses it with DI
water
Monthly
Clean Bulk Injection
Chamber on page 191
Decontaminates the sample
injection chamber with
ethanol
As needed
Prepare for Aseptic Sort
on page 193
Decontaminates the complete
sheath path and sample path
with ethanol and rinses it with
DI water
When needed before
aseptic sorting
Some of the internal cleaning modes require that you turn off the stream.
Remember to open the flow cell access door to block the lasers before you
turn off the stream. Running the lasers without the stream can degrade the
performance of the cuvette flow cell.
To Next Page
Loading...
