Quadra 4 Operator Manual
Tomtec
131
1. Under the options icon you will find the magnetic separation device option. Select
this to turn on the magnets at position 1. At this time you would like the beads to
settle toward the bottom of the nest for a full two minutes. The
recommendation at this time would be to wash your tips in the ultrasonic bath
for two minutes or to wash the tips and add a timed pause for the
remainder of the time (if it takes :50 sec to wash the tips, set a timed pause 70
seconds.
2. To yield the full 120 second wait time). Another method would be to lock-out that
position for 120 seconds (located under options). After the 2-minute pause the
supernatant needs to be transferred to the assay plate.
To accomplish this Aspirate 20uL from position 1, (with the magnets still on)
Turn the magnets off, and then Dispense 20uL into assay plate in 4. At this time you
should wash the tips to avoid contaminating the other reagents. The final step is to rinse
the magnetic particles with ETOH. To do this, Aspirate 15uL from the ETOH reservoir in
6, Dispense 15uL into position 1, and Mix 12-15uL in 1 a few times. To avoid removing
the magnetic particles, turn the magnets back on. You may wish to wait a few moments
to allow the beads to travel to the bottom of the wells so a pause of a few seconds can
be added, if not Aspirate 15uL from position 1 and dispense it into the waste station at
position 5. The protocol has now finished 1 plate. You can’t forget that the next 19
plates must be done in the exact same way, so wash the tips one last time to
prepare them for the next go around. The final touch is to place the end loop in its’
proper location. To do this, go to the beginning of the transfer and select the second
step, the "end loop" command. Once this step is highlighted, go over to the
right side of the menu and select the move down icon. The step has now gone
from being step two to step three. Keep pressing the move down icon until the
end loop is the last step. The protocol is now completed and ready to run.
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