Quadra 4 Operator Manual
Tomtec
97
Volume
Volume is the amount of liquid (in µL for plates and mL for reservoirs) that a single well
or reservoir can hold.
Well Rate
Once Top Steps, Bottom Steps, and Volume are entered, it is possible to estimate the
well rate by clicking on the button next to the field. This field is user editable, and it is
advised that you test, and calibrate the well rate manually. The application will only
provide an estimate.
4.3 The Fixture Panel
Fixtures are easier than plates to calibrate because there is only one setting,
the Calibration Height. This is, by definition, the height at which the tips meet the
fixture, without pinning the fixture to the stage.
There are several fixtures, preinstalled into the software, that are not user-
editable. The only attribute the user may edit is the calibration height. Any new
fixtures, or custom fixtures are user editable, and must specify whether or not they can
operate with a Quadra Stacker, whether or not they can operate with a Tip Feeder, and
whether they can be pipette from/to.
Tip jigs are fixtures that require no user intervention. All tip jigs have a load height of
zero. If any value is inserted into this field, it will override the load height set in the
system parameters function. The same is true for the shuck height.
After setting the Load Height, if you are unsatisfied with the result, you may
click the Reset Calibration button to return to the original value, only before you
save. The application will prompt you to save any changes when you switch records, or
close the application.
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