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TomTec QUADRA 4 - Pipetting Tip #5 Recommendations

TomTec QUADRA 4
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Quadra 4 Operator Manual
Tomtec
189
b. Using 2µL of blow out air will improve the Cv of the spot.
c. Dispense the 0.5µL aliquot with the tips in free air above the wells. Then move the
tips down to the touch off height and dispense the 2µL of blow out air.
d. Do not try to rinse the tip exterior as described in Tip # 3. If the 0.5µL is moved away
from the orifice, it will be lost without the supporting liquid above. If rinsed with the
0.5µL at the orifice the rinse water will capture it.
5.0 Tip #5
When mixing reagents in the well, aspirate from the bottom of the well and dispense
above the final liquid level. This will turn the liquid over. It is recommended that you mix
with 70-75% of the well volume.

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