Quadra 4 Operator Manual
Tomtec
201
11.0 Calibration Instructions for 450 µL Head
11.1 Definitions
CALIBRATION
Is defined as the process by which the correlation between instrument response
and actual value of a measured parameter is determined.
CALIBRATION CURVE
Is defined as the curve which plots the amount of liquid dispensed versus the
expected amount. Also known as a Standard Curve.
INACCURACY
Is defined as the difference between the DISPENSED volume and the
NOMINAL, or expected, volume to be dispensed. Also known as SYSTEMATIC
ERROR.
11.2 Liquid Used For Calibration Curve Set Volumes
The Calibration Liquid for a “Fluid Class of Water” should be distilled or de-
ionized water conforming to grade 3 as specified in ISO 3696, degassed or air-
equilibrated. The water shall be at room temperature (20º C).
12.0 Ambient Calibration Conditions
The following Ambient Calibration Conditions should be complied with:
AIR TEMPERATURE 25 C to 35 C (constant 0.5 C)
RELATIVE HUMIDITY 50 % to 65 %
12.1 Importing an Existing Calibration Curve
a. Open the “QUADRA 4
COMMAND SYSTEM” program.
b. In the upper menu bar select “UTILITIES”.
c. On the pull down menu under “UTILITIES” select “IMPORT DATA”.
d. Select the USB drive and select “WATER (XX-XX-XX).csv”.
e. A popup window will ask for LIQUID TYPE - type in “WATER” - Click “OK”.
f. Checking the “SHOW REFERENCE CURVE” box will graphically display the
new calibration curve.
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