TomTec QUADRA 4 - Verification of Calibration Curve Procedure; Evaporation

Quadra 4 Operator Manual
Tomtec
193
2.2 Verification of Calibration Curve Procedure - label each Weighing
Vessel with the sample number - Consisting of the volume to be calibrated and the
pipetting number (1, 2, 3) i.e., 5-1, 5-2, 5-3, … for each of the 10 SET VOLUMES listed
below required for verification of the calibration curve.
SET VOLUME 1
ST
PIPETTING 2
ND
PIPETTING 3
RD
PIPETTING
10µL 10-1 10-2 10-3
25µL 25-1 25-2 25-3
50µL 50-1 50-2 50-3
75µL 75-1 75-2 75-3
100µL 100-1 100-2 100-3
125µL 125-1 125-2 125-3
150µL 150-1 150-2 150-3
250µL 250-1 250-2 250-3
350µL 350-1 350-2 350-3
400µL 400-1 400-2 400-3
Using the 4 Place Balance – Record tare weight of each Weighing Vessel.
3.0 Evaporation
For small volumes, below 50µL, errors due to evaporation of the Calibration Liquid
during weighing should be taken into consideration. To keep the error, due to
evaporation, as small as possible the following should be considered:
A balance with the appropriate accessories such as an evaporation trap.
Pipetting to weighing Cycle Time.