Quadra 4 Operator Manual
Tomtec
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met, the csv file is moved into a sub directory named “Completed” and the Quadra will
no longer check barcodes.
In some instances, it may be necessary to have additional steps within the protocol
when processing the Mirror Racks. Any additional steps in the protocol which are not
related to the standard mirror rack checking should have the "Disable Barcode Reading"
option checked. This will cause that step to be ignored by the Mirror Rack checking
code.
Also, it is important to note that the barcode will only be read correctly if the stage is all
the way down before the step starts. In some cases it might be necessary to set the
option to "Drop Stage after Dispense" in the step BEFORE the step which checks the
barcode.
1.3 Quadra 4 - Getting Started
Creating a stacker program on a Quadra 4 is a multi-step process and there are
certain rules that apply. The objective is to create a smooth running program
that can be repeated on demand. The real advantage of automation is to create
a specific protocol, that will run precisely the same, every time. This allows small
optimizing modifications that will be faithfully reproduced to enhance the end results.
Once the program is created, anyone can execute it. The stackers are loaded in the
defined order of the program and then executed precisely the same every time the
protocol is run.
Not every Shuttle Station can access all of the stackers. Shuttle station 2 is the most
flexible. It can access all four stackers. Station 1 can access the Left Rear
Stacker (LRS) and the Left Front Stacker (LFS). Station 3 can access the Right
Rear Stackers (RRS) and the right front stacker (RFS). Station 5 can access the LFS
and RFS. Stations 4 and 6 can only access a single stacker each, the LFS and the RFS
respectively. Thus, they are the least flexible. Therefore, they are commonly used for
fixed functions, such as the Ultrasonic Tip Wash Station or a vacuum box. They are also
useful for a common reservoir that is used through out the protocol.
While each pipetting protocol may be different, in general terms, it is effective to use the
left rear stacker for the incoming source plates, or reservoirs and the right rear stacker
for the destination plates. In this manner at the end of the sequence, the source plates,
or reservoirs may be restacked for the next set of destination plates. The tip boxes are
then interspersed, depending on whether they are to be assigned and reused with the
specific sources or not. A distinct advantage of the Quadra 4 is the ability to make a
non-contact dispense. The target method may be used to make a clean dispense at the
top of the well, without touching the contents of the receiving plate. This allows
assigning a rack of tips for use with specific reagents, without fear of cross
contamination.
As the pipetting protocol become more complex, moving microplates, reservoirs and
tips in and out of the stackers to the shuttle becomes more challenging. While
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