Quadra 4 Operator Manual
Tomtec
177
6.0 Bubbling
This method is to aspirate the total tip volume of air (at the clear height) then
dispense this air to the bottom of the well using speed 3. This can be a very
effective method of mixing. It may not be suitable for those reagents containing a
detergent or other surfactant due to foaming.
7.0 Injection
This method is restricted to the air displacement pipettor heads that have the ability
to blow pressurized air through the tip orifices.
The desired volume is aspirated in the tips. The tips are moved above the liquid
surface of the well. A timed dispense function is made, applying air pressure to the
tips, ejecting the tip contents. The three parameters controlling this function are the
volume in the tip, the dispense height above the liquid surface, and the applied air
pressure.
These must be controlled to keep below the splashing level. Part of the liquid
volume may be dispensed with piston motion, using air pressure to dispense the
last. The air pressure is set by the black knob on the side that regulates the air
pressure in the internal air source. 1 to 2psi is recommended as a starting point.
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