TomTec QUADRA 4 - Creating a New Calibration Curve with Water

Quadra 4 Operator Manual
Tomtec
196
8.0 Creating a New Calibration Curve With Water
The “CALIBRATION CURVE” procedure consists of pipetting a set volume of the
Calibration Liquid and creating a correction curve to increase Pipetting Head Accuracy.
The procedure uses 20 pipetted set volumes pipetted 3 times.
For each set volume, the Expected Weight is compared to the Dispensed Weight as a
% ERROR using the following formula:
(EXPECTED WEIGHT / PER WELL) – (DISPENSED WEIGHT / 96)
------------------------------------------------------------------------------------ X 100 = % ERROR
(EXPECTED WEIGHT / PER WELL)
NOTES:
1. 1 GRAM = 1mL
2. Tips are pre-wetted prior to performing the following Calibrations.
Place the Tip Loading Jig Cat # 320-50) with a rack of Quadra Pipetting Tips
(Cat # 196-205) on position 1 of the shuttle.
Load the rack of Quadra Pipetting Tips using the “LOAD TIPS” program.
Place a Plate / Reservoir Nest (Cat. No. 320-30) with a Polypropylene Reagent
Reservoir (Cat. No. 196-130-00) containing the Calibration Liquid onto the shuttle,
Position 6.
Place a Plate / Reservoir Nest (Cat. No. 320-30) onto the Shuttle, Position 5.
Run the “PRE WET” program.
3. The time between dispensing and weighing should be minimized to avoid errors due
to evaporation.
4. The average % Error of the 3 weights for each set volume is used in creating the
Calibration Curve data points.
5 Tomtec provides a spreadsheet to calculate the % ERROR. It also calculates the
mean, standard deviation, and CV of the specific samples that are entered for each
volume.