TomTec QUADRA 4 - Page 197

Quadra 4 Operator Manual
Tomtec
197
CREATING CALIBRATION CURVE DATA POINTS
Place the tared Weighing Vessel labeled 5-1 onto the Plate / Reservoir Nest at Position
5 of the Shuttle.
Run the “CAL 5L” program to dispense 5.0L of the Calibration Liquid into the tared
Weighing Vessel labeled 5-1.
After the Calibration Liquid is Dispensed, immediately obtain the gross weight of the
sample and Weighing Vessel labeled 5-1. Record the weight onto the supplied
spreadsheet.
Place the tared Weighing Vessel labeled 5-2 onto the Plate / Reservoir Nest at Position
6 of the Shuttle.
Run the “CAL 5L” program to dispense 5.0L of the Calibration Liquid into the tared
Weighing Vessel labeled 5-2.
After the Calibration Liquid is Dispensed, immediately obtain the gross weight of the
sample and Weighing Vessel labeled 5-2. Record the weight onto the supplied
spreadsheet.
Place the tared Weighing Vessel labeled 5-3 onto the Plate / Reservoir Nest at Position
6 of the Shuttle.
Run the “CAL 5L” program to dispense 5.0L of the Calibration Liquid into the tared
Weighing Vessel labeled 5-3.
After the Calibration Liquid is Dispensed, immediately obtain the gross weight of the
sample and Weighing Vessel labeled 5-3. Record the weight onto the supplied
spreadsheet.
Continue this routine using the supplied programs for the remaining 15 SET VOLUMES
(10L to 450L ) doing 3 pipettings of each volume.
Shuck and dispose of the QUADRA Pipetting Tips using the “SHUCK TIPS” program.