Quadra 4 Operator Manual
Tomtec
75
Mix Cycles/Dilution
The number of mix cycles(0 to 99) per dilution. A zero(0) value will eliminate the mix
function.
Aspirate Speed
See Aspirate Speed in the Aspirate.
Dispense Speed
See Dispense Speed in the Dispense Function.
Row/Column Offset
The location for Dilution 1. All subsequent dilutions will follow left to right or top to
bottom of the plate, one row at a time. See Quad & Row/Column Offset in Stage
Operations for more details.
Ripple Volumes
This is a convenience when setting up the serial dilution to allow the same volume to
automatically be set for multiple successive dilutions. For example, if Ripple Volumes
is checked and I change Dil. 2 Mix volume, Dil. 3 Mix volume and down will
automatically be set to the same volume. Dil. 1 Mix Volume would be unaffected. Ripple
Volumes is a design time tool only. It has no bearing when the program is actually
running.
Dilution # Mix
The mix volume for each dilution performed.
Dilution # Xfer Out
The transfer volume from current dilution to the next. If a transfer out volume is specified
for the last dilution, that volume will be in the tips at the completion of the function.
3.26 Shaker Nest
A stacker accessible nest that has an electronic oscillating mechanism that can
be turned on and off programmatically in the Quadra. The oscillation amplitude is
controlled by a dial on the Shaker Nest.
Conditions
You must have a shaker nest on the specified shuttle position, properly connected to
the Quadra in order for this function to work.