TomTec QUADRA 4 - Page 199

Quadra 4 Operator Manual
Tomtec
199
CREATING VERIFICATION OF THE CALIBRATION CURVE DATA POINTS
Place the tared Weighing Vessel labeled 10-1 onto the Plate / Reservoir Nest at
Position 5 of the Shuttle.
Run the “VER 10L” program to dispense 10.0L of the Calibration Liquid into the
tared Weighing Vessel labeled 10-1.
After the Calibration Liquid is Dispensed, immediately obtain the gross weight of the
sample and Weighing Vessel labeled 10-1. Record the weight onto the supplied
spreadsheet.
Place the tared Weighing Vessel labeled 10-2 onto the Plate / Reservoir Nest at
Position 6 of the Shuttle.
Run the “VER 10L” program to dispense 10.0L of the Calibration Liquid into the
tared Weighing Vessel labeled 10-2.
After the Calibration Liquid is Dispensed, immediately obtain the gross weight of the
sample and Weighing Vessel labeled 10-2. Record the weight onto the supplied
spreadsheet.
Place the tared Weighing Vessel labeled 10-3 onto the Plate / Reservoir Nest at
Position 6 of the Shuttle.
Run the “VER 10L” program to dispense 10.0L of the Calibration Liquid into the
tared Weighing Vessel labeled 10-3.
After the Calibration Liquid is Dispensed, immediately obtain the gross weight of the
sample and Weighing Vessel labeled 10-3. Record the weight onto the supplied
spreadsheet.
Continue this routine using the supplied programs for the remaining 9 SET VOLUMES
(25L to 350L ) doing 3 pipettings of each volume.
Shuck and dispose of the QUADRA Pipetting Tips using the “SHUCK TIPS” program