Quadra 4 Operator Manual
Tomtec
55
Dispense Speed
The Dispense Speed is the rate at which the tips will pipette liquid. This can be a
value between 1 and 4, 4 being the fastest. By default, 3 is automatically
selected.
Dispense Height
Dispense Height is the height at which the tips will dispense from within the well.
This allows you to dispense at the appropriate height to wells without creating air
pockets within the wells.
Selecting “Clear Position”, “Top”, or “Near Bottom” will use the height values set
within the database for the plate being used. If a different value is required, this
can be set in the “Advanced Settings”.
Advanced Settings
Calibration Table
The Calibration Table drop down menu consists of user defined pump data for
the liquid type used. See documentation on distribution CD.
Blow Out Volume
Blow Out Volume is simply the amount of air (in µL) at the end of your Dispense
Step in order to flush the tips of all remaining liquid.
Blow Out Height
Blow Out Height is the height at which the tips will blow out the remaining
substance from within the well.
Touch Off
While Dispensing liquid into the plates, due to the surface tension at the tip
orifice, drops may form and remain on the tips when dispensing has concluded.
To accurately pipette the correct amount of liquid into the wells, these drops must
be captured. Using the ‘Touch Off’ is one method of capturing hanging
drops remaining on the tips. By setting the correct values within the Plate
Database, you can have the tips contact the side of the wells in order to release
the drops into the wells.
The Touch Off will occur at the height at which the Dispense or Blow Out has
ended. For example, if Liquid Following has been enabled, the Touch Off will
occur at the height at which the stage has come to a rest, otherwise it
will occur at the height specified by the ‘Dispense Height’ value.
Dip Tips
Dip Tips is another method of capturing hanging drops. Dip Tips is a
vertical Touch Off. What this function accomplishes is capturing drops held
on the tips by placing the tips in very close proximity to the liquid that has already
been dispensed into the wells.
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