SYSMEX CA-500 - Analysis Protocol

5.11.10 Analysis Protocol
(1) PT
Sample Aspiration 50 µL
First Reagent 180 sec
Reagent volume 1 PT 100 µL
Rinse 11 100 µL
Detection Activity-PT
Coag. detection point 50%
Sensitivity Low Sensitivity
Max. detection time 100 sec
(2) APTT
Sample Aspiration 50 µL
First Reagent 60 sec
Reagent volume 5 APTT 50 µL
Rinse 11 50 µL
Second Reagent 240 sec *
Reagent volume 7 CaCl2 50 µL
Rinse 11 50 µL
Detection Activity-APTT
Coag. detection point 50%
Sensitivity Low Sensitivity
Max. detection time 190 sec
*
NOTE: This depends on the APTT Incubation time setting (Service Setting).
Japanese market: 180 sec
(3) Fbg
Sample Aspiration 10 µL
Dilution volume 12 Buffer 90 µL
First Reagent 180 sec
Reagent volume 3 Fbg 50 µL
Rinse 11 50 µL
Detection Activity-Fbg
Coag. detection point 50%
Sensitivity High Sensitivity
Max. detection time 100 sec
(4) TT
Sample Aspiration 100 µL
First Reagent 120 sec
Reagent volume 9 TT 50 µL
Rinse 11 50 µL
Detection Activity-others
Coag. detection point 50%
Sensitivity Low Sensitivity
Max. detection time 190 sec
CA-500 Series S/M 5-27 Revised December 2001 8