Quadra 4 Operator Manual
Tomtec
169
ELECTRONIC (Z) OFFSET PROCEDURE
1) The DMV pipette head should be installed and aligned in the Quadra 4 instrument.
2) Turn on Quadra 4 POWER
3) Connect and Initialize the Quadra 4
4) Place actual Tip Load Fixture under pipetting head
5) Place proper volume pipette tip rack in Tip Load Fixture
6) Select Utilities
7) Select Pump
8) Select proper volume Tips from drop down menu
9) Select Load Tips
10) Remove pipette tip rack box
11) Close out Utility Window
12) Select System
13) Select set system parameters
14) Enter Password (41560)
15) Select Board A – X,Y,Z - Stage
16) Select Stage z delta alignment utility
17) Select zero Z default
18) Enter Stage Height of 1455
19) Move stage to 1455
20) Evaluate gap between pipette tips and top plate of tip load fixture
21) Jog stage up or down until there is an approximately .005” gap
22) Select set new z delta
23) Select OK
24) Select Initialize
25) Use system utility to test new z delta offset
26) Select OK
27) Select initialize stage
28) Select system utilities
29) Set Stage Height to 1455
30) Move stage to 1455 steps
31) Check for proper stage height
(If offset needs additional adjustment - repeat steps 13 – 23)
32) Select Initialize
33) Replace pipette tip rack box on tip load fixture
34) Shuck Tips
35) Close Utility Window
36) Select OK
37) Select apply changes
38) Select OK
39) Select Reconnect and Initialize Quadra 4
40) Connect and Initialize Quadra 4
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