Quadra 4 Operator Manual
Tomtec
6
2.2 Adjusting Tip Load Switch ............................................................................. 165
3.0 Quadra 4 Positive Displacement Pipettor Heads .................................................... 166
4.0 Quadra 4 Disposable Multi Volume Head (DMV) .................................................... 167
5.0 Setting the Zero Volume Adjustment for Air Displacement Pipettor Heads ............ 170
6.0 Quadra Shuttle ........................................................................................................ 172
7.0 Stackers .................................................................................................................. 172
8.0 Quadra Stage .......................................................................................................... 173
Section V - Suggested Pipetting Methods & Recommendations ........ 175
1.0 Aspirating ................................................................................................................ 175
2.0 Pipeline Pipetting .................................................................................................... 175
3.0 Dispensing .............................................................................................................. 176
4.0 Mixing ...................................................................................................................... 176
5.0 Titration ................................................................................................................... 176
6.0 Bubbling .................................................................................................................. 177
7.0 Injection ................................................................................................................... 177
Section VI - Pipetting with the Positive Displacement Pipettor Head . 178
1.0 Pipetting 0.5µl with Positive Displacement .............................................................. 178
2.0 Dispensing 0.5µl spots - Aqueous ........................................................................... 178
3.0 Dispensing 0.5µl spots – DMSO ............................................................................. 179
4.0 Protocol Verification ................................................................................................ 179
Section VII - Bioanalytical Applications ................................................. 180
1.0 Aqueous and Organic Pipetting .............................................................................. 180
2.0 Protein Precipitation, Liquid/Liquid, And SPE ......................................................... 180
3.0 Protocol Or Program Security ................................................................................. 181
4.0 Calibration For The Quadra 4 ................................................................................. 181
Section VIII - Quadra 4 Accessories ....................................................... 184
1.0 Custom Reservoirs.................................................................................................. 184
1.1 Keeping Participate Matter in Suspension ..................................................... 184
1.2 Stirring Reservoir ........................................................................................... 184
1.3 Constant Level Reservoir .............................................................................. 185
1.4 Shaker Nest................................................................................................... 185
1.5 Live Bottom Reservoir ................................................................................... 185
1.6 Thermal Retention Reservoir ........................................................................ 185
1.7 Peltier Nest .................................................................................................... 186
1.8 SPE Vacuum Box .......................................................................................... 186
1.9 Filtration System for the Quadra 4 ................................................................. 186
1.10 Auto Regulated Mini Vacuum Trap .............................................................. 187
1.11 Fully Automated Filtration System ............................................................... 187
Section IX - Pipetting Recommendations .............................................. 188
1.0 Tip# 1 ........................................................................................................... 188
2.0 Tip #2 ........................................................................................................... 188
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