Quadra 4 Operator Manual
Tomtec
7
3.0 Tip #3 ........................................................................................................... 188
4.0 Tip #4 ........................................................................................................... 188
5.0 Tip #5 ........................................................................................................... 189
Section X – Calibration of the Quadra 4
®
450µL ................................... 190
1.0 Equipment Required ............................................................................................... 191
2.0 Weighing Vessels .................................................................................................... 191
2.1 Calibration Curve Procedure ......................................................................... 192
2.2 Verification of Calibration Curve Procedure .................................................. 193
3.0 Evaporation ......................................................................................................... 193
4.0 Pipetting To Weighing Cycle Time ...................................................................... 194
5.0 Setup Procedure ................................................................................................. 194
6.0 Adding the New Calibration Curve into the Quadra Command System from Data
Points 194
7.0 “Calibration Template – Q4” Spreadsheet .......................................................... 195
8.0 Creating a New Calibration Curve With Water .................................................... 196
9.0 Creating a Verification Curve .............................................................................. 198
10.0 “Calibration Template” For the Verification Curve ............................................... 200
11.0 Calibration Instructions for 450 µL Head ............................................................. 201
11.1 Definitions ................................................................................................. 201
11.2 Liquid Used For Calibration Curve Set Volumes ................................................. 201
12.0 Ambient Calibration Conditions ........................................................................... 201
12.1 Importing an Existing Calibration Curve .................................................... 201
Section XI - Some Properties of Liquids That Affect Pipetting Accuracy
................................................................................................................... 202
1.0 The relationship between liquid and gaseous states of a solvent have an effect on
pipetting ......................................................................................................................... 202
2.0 Additional properties of liquids that might have an effect on pipetting ................... 202
3.0 To compensate for these properties of liquids, the aspirate and dispense steps have
options to improve performance ..................................................................................... 203
Section XII - Head Removal, Installation and Alignment ...................... 204
1.0 Top Rear View ......................................................................................................... 204
2.0 Top Front View (Open Panel) .................................................................................. 205
3.0 Head Installation ...................................................................................................... 206
5.0 450µL Backfill Head Back View .............................................................................. 208
6.0 Tip Block Assembly ................................................................................................. 209
7.0 55µL SV Head ......................................................................................................... 210
8.0 384 SV Tip and Seal Assembly ............................................................................... 211
Section XIII – Routine & Preventive Maintenance by Operator ........... 212
Maintenance and Schedule
....................................................................................... 212
Routine & Preventive Maintenance by Operator ............................................................ 212
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