2.2 Measurement Precautions
HA-8190V 2-11
2.2.5 Samples
Anticoagulant for whole blood
Use one of the following anticoagulants: heparin, EDTA-2Na, EDTA-2K, EDTA-3K or NaF.
Never measure samples that contain iodoacetic acid as an anticoagulant in order to prevent degradation of the column.
When storing whole blood samples:
Refrigerate whole blood samples at a temperature between 2°C and 8°C. Samples can be stored in stable condition for
3 to 4 days to obtain A1c values.
TAKE THE UTMOST CARE WHEN HANDLING BLOOD. This instrument uses blood as
sample. Blood may be contaminated by pathogenic microbes that can cause infectious
diseases. Improper handling of blood may cause infection to the user or other individuals by
pathogenic microbes.
Discard used samples in accordance with local regulations for biohazardous waste.
IMPORTANT:
When using hemolysis samples:
If left at room temperature, hemolysis samples gradually degenerate, which will affect measurement.
Promptly measure hemolysis samples without leaving them at room temperature for any extended periods
of time.
Hemoglobin concentration of hemolysis samples
Prepare hemolysis samples so that the total area of the measurement result is between 30,000 to 60,000
counts. If the concentration does not fall within this range, an error may occur or the reproducibility may
become poor. If this happens, change the dilution ratio appropriately to adjust the concentration before
performing a measurement again. (This instrument dilutes whole blood 101 times before measuring it.)
Samples without plasma
Sample racks for whole blood cannot be used to measure samples from which plasma has been removed.
With the whole blood racks, samples without plasma cannot be diluted to the proper hemoglobin
concentration and consequently produces inaccurate measurement results. To measure such samples,
dilute them with DILUENT 90 and measure as hemolysis samples.
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