Chapter 2 Measurement
HA-8190V 2-10
2.2.4 Column
Wear protective gloves to prevent exposure to pathogenic microbes when replacing the
column.
Discard used columns in accordance with local regulations for biohazardous waste.
IMPORTANT:
Use only columns specified for the instrument.
ARKRAY provides “COLUMN UNIT 90” as columns specifically for the HA-8190V. Before use, read the
package insert that comes with the column and observe all handling instructions.
When storing the columns:
• Store unopened column units at a temperature between 3°C and 25°C. It is recommended to keep the
column refrigerated. Do not freeze.
• Do not leave the instrument with the column installed for extended periods of time. If the instrument is
not to be used for one week or more, perform the required maintenance tasks and then store the
removed column with both ends sealed at a temperature between 3°C and 25°C (see “4.6.1. Preparing
the Instrument Before Extended Periods of Disuse” on page 4-55). It is recommended to keep the
column refrigerated. Do not freeze. If not properly stored, the filler may dry beyond a level of practical
use.
Observe expiration dates.
Do not use columns beyond their expiration date. The expiration date is written on both the box and the
label attached to the OUT side of the column.
Install the column in the correct direction.
Check the IN and OUT sides of the column when installing it in the instrument. Connect the IN side of the
column to the IN column tube, and the OUT side, to the OUT column tube of the instrument.
Keep the sealing screws for future use.
Keep the sealing screws removed from new columns in the accessory case. These screws should be
reused if the instrument is not to be used for extended periods of time.
Do NOT introduce anything other than eluents into the column.
Do not introduce anything other than eluents into the column. The introduction of surfactant, fat or
water-insoluble materials can alter separation, making it impossible for the instrument to measure samples.
Even a trace amount of foreign matter can accumulate inside the column and reduce its service-life
significantly. (Distilled water or air can also cause the elution conditions to change and produce inaccurate
measurement results.)
Do NOT disassemble the column.
Trouble may occur or inaccurate measurement results may be obtained, if the column is disassembled.
Do NOT subject the column to shocks or vibrations.
Trouble may occur or inaccurate measurement results may be obtained, if the column is subjected to strong
impacts or vibrations.
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