Preoperative Sequestration (PPP and PRP)
X
TRA Operator’s Manual 20077/021 US 10-3
fluid in the PPP and/or PRP bags includes plasma, platelets, and other formed elements, and may
include some RBC’s.
The plasma, platelets, and red cells may be transfused during or after the surgical procedure under
the direction of the physician. The volume of plasma and platelet yields obtained during the
sequestration procedure are dependent on the volume of blood withdrawn from the patient, as well as
on the patient’s hematocrit and platelet count at the beginning of the procedure.
The company recommends harvesting approximately 20% of the circulating platelets. Fill speeds may
affect platelet yields. Decreasing the fill speed increases the percentage of platelets harvested and
conversely, increasing the fill speed decreases the percentage of platelets harvested.
Sequestration Set
The Sequestration Set is available for processing whole blood previously collected into blood bags.
When whole blood from the patient is drawn into collection bags prior to the preoperative
sequestration process, blood flows from the patient by gravity into collection bags. These bags
(obtained from the blood bank) contain citrate in a ratio of 1:5 - 1:10 (one drop of citrate to five drops
of blood to one drop of citrate to ten drops of blood).
A Sequestration Set (see Figure 10-1) is used in conjunction with the X
TRA Bowl Set and Blood
Collection Reservoir. The X
TRA Sequestration set includes:
• Spike connector
• Blood bag connection line
• 4-way adapter with two sequestration lines respectively identified by a yellow and an orange
clamp
• Two 1-liter transfer bags for plasma and/or platelet collection.
Figure 10-1 Sequestration Set
The spike connector, when connected to the blood inlet line, allows access to the whole blood bag.
The blood bag connection line allows connection of the whole blood bag, containing blood drawn from
the patient to the bowl set.
CAUTION
When collecting PRP, LivaNova recommends the following precautions be
taken to insure that the PRP product is not contaminated:
• Use sterile techniques when setting up the X
TRA
disposables.
• Thoroughly clean and disinfect the donation site.
• Use sterile techniques whenever handling the PRP product.
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