Preoperative Sequestration (PPP and PRP)
X
TRA Operator’s Manual 20077/021 US 10-19
Finishing Preoperative Separation and Preparing for Blood Recovery
To finish preoperative sequestration and prepare for blood recovery, do the following:
1. Remove any air from the PRP bags.
2. Clamp both PRP bags. Detach the PRP bags from the plasma sequestration lines.
3. using the caps and plugs provided, cap and plug the PRP bags and lines. This will decrease the
chance of contamination and any bio-hazard caused by dripping.
4. Reinfuse autologous plasma according to hospital guidelines and/or AABB standards.
5. Unclamp the inlet tube to the waste bag.
6. Clamp the blood inlet line from the whole blood bag to isolate the patient line and unclamp the
line from the reservoir.
7. Connect the Wash line to the wash solution.
a. Hang the wash solution bags on the lower hooks of the IV pole.
b. Using aseptic technique, spike each Wash line into a bag of wash solution. Unclamp at least
one line.
8. If you are using a new reinfusion bag:
a. Remove the cap from the reinfusion bag.
b. Remove the cap from the tubing line and twist connect to the reinfusion bag
c. Hang the new reinfusion bag.
WARNING
To reduce risk of air embolism, remove all air from the plasma bags
before handing the bag over for reinfusion.
WARNING
Do not reinfuse under pressure (i.e., do not use a blood pressure cuff
on the RBC or plasma/PRP bags). Reinfusion under pressure could
lead to air embolism.
WARNING
Non-red cell components (e.g., platelet rich/platelet poor plasma
intended for transfusion and platelet rich/platelet poor plasma)
shall be used or applied before the patient leaves the operating
room or clinical procedure area.
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American Association of Blood Banks.
Standards for Perioperative Autologous Blood
Collection and Administration
. 3rd Edition. Bethesda, MD. 2007 Reference Standard
5.1.8 (Handling, Storage, Transportation)
WARNING
Do not reinfuse the PRP back to the patient if the XTRA fails to
operate as intended.
CAUTION
Make sure that the waste line is unclamped before starting blood salvage
processing.