Preoperative Sequestration (PPP and PRP)
10-4
20077/021 US XTRA Operator’s Manual
The 4-way adapter is inserted between the outlet tubing from the centrifuge bowl and the waste bag;
its two sequestration lines are connected to the blood bags, for plasma (yellow clamp) and platelet
collection (orange clamp). During a preoperative sequestration, the clamps are used to direct the flow
of plasma and platelets into the collection bags.
Order Guide
The following is the disposable set that must be used with XTRA for sequestration:
Before You Begin
The XTRA enables you to conduct preoperative sequestration and then convert easily and quickly to
intraoperative blood recovery. This section explains how to:
• Set up and load disposables for sequestration
• Process blood for sequestration
• Convert to intraoperative blood recovery
Before beginning preoperative sequestration, note the following warnings and cautions.
Catalogue No. Product Designation Product Description
04015 Sequestration Set X The product is used for separation of Platelet-Poor
Plasma (PPP) and Platelet-Rich Plasma (PRP)
from the patient’s whole blood collected into
transfer bags.
Table 10-1
WARNING
Whole blood must be anticoagulated as it is collected into bags
containing appropriate anticoagulant for plasma sequestration.
Inadequate anticoagulation may result in clotting, interfering with
the processing of the blood products.
WARNING
If plasma is being collected for transfusion, it must be transfused
before the patient leaves the operating room or clinical procedure
area.
WARNING
Do not over anticoagulate collected blood. Plasma Sequestration has
no Wash cycle to remove excess anticoagulant. A part of the
anticoagulant used will be returned to the patient.
WARNING
Do not overfill PPP and PRP bags. Overfilling may cause back
pressure that would cause fluid to exit through the bowl seal.
WARNING
Do not reinfuse the PRP back to the patient if the XTRA fails to
operate as intended.