10 - 26
HAPSITE ER Operating Manual
End Mass. . . . . . . . . . . . . . . . . . . . . The mass at which the Mass Spectrometer
will end a scan. The end mass can be set
from 1-300 amu. This value must be larger
than the start mass.
NOTE: End the scan at least 2 amu above any mass used for compound
identification. However, do not increase the end mass higher than
necessary, as this will increase the scan time and a lower number of scans
will be collected.
Dwell Time. . . . . . . . . . . . . . . . . . . . The Dwell Time is the length of time the
Mass Spectrometer will sample data at each
sampling point. The longer the Dwell Time,
the better the signal to noise ratio of the
analyte.
Run Time . . . . . . . . . . . . . . . . . . . . . The time span of the method from start to
finish.
10.9 SIM Page
Selected Ion Monitoring (SIM) scans a set of specific masses to increase the
sensitivity for known compounds. Figure 10-31 displays the SIM page.
10.9.1 SIM for Analyze
Each set has a Begin Time and an End Time which must be entered when
programming the Set. An optional Name can also be entered at this point. After
entering the times, the mass fragments for the compound can be entered into the
Mass column. As the mass fragments are entered, the Round Trip Time is
automatically calculated and entered in the Scan Sets grid.