Preoperative Sequestration (PPP and PRP)
10-14
20077/021 US XTRA Operator’s Manual
When the Spill PPP sub-phase begins, the centrifuge speed starts decreasing slowly until it reaches the
pre-set Spill PPP speed. During this time the message “Automatic pause - Please wait for centrifuge
deceleration” is displayed in the message area.
At this stage, the pump remains paused for one minute to improve blood sedimentation. The
message “Automatic Pause - Press Play button to start pump” is displayed in the message
area. Touching the Play button will interrupt the pause and start the pump.
At the end of the automatic pause period, the pump resumes its rotation at the set speed until the set
PPP Spill volume is transferred to the bowl.
At this point, if processing with the PRP2 protocol, the message “Close PPP line. Open PRP line. Then
press Play button” is displayed in the message area. Performing these actions allows PRP to be
collected in a separate bag.
5. If processing with the PRP2 protocol, clamp the PPP line (yellow clamp), open the PRP line
(orange clamp), then touch the Play button, as prompted.
The Spill PRP sub-phase begins, as indicated in the title bar of the processing displet.
During the Spill PRP sub-phase, the volume of PRP spilled into the collection bag, as well as the total
volume of PPP and PRP spilled, is displayed on the screen and constantly updated by the system’s
software. The desired total volume of PRP to spill, and the spill speed, can be adjusted from the
processing displet.
With the pump stopped, the system enters a second automatic pause period to again compress the
buffy coat and improve the blood sedimentation. The message “Automatic Pause - Press Play button to
start pump” is displayed in the message area. Touching the Play button will interrupt the pause and
start the pump.
At the end of this second automatic pause period, the pump resumes its rotation at the set speed until
the set PRP Spill volume is transferred to the bowl.
Note: To get better platelets yield, it is advisable not to vary the pump flow during the PRP Spill
phase.
6. When operating in both the Automatic mode and in Manual mode, the pump will stop and
processing will automatically continue to the Empty phase as soon as the set PRP volume has
been spilled.
Note: It is possible to manually advance phases while operating in the Automatic mode. Touching
the Pause button from a Processing Screen will stop the pump and display phase buttons in the button
bar. The operator may touch the appropriate phase button (Spill or Empty) to switch to that phase, or
touch the Play button to continue processing in the current phase.
The Empty Phase
When the Empty phase begins, the centrifuge and pump stop and the processing displet changes to
reflect that the Empty phase is in progress (see Figure 10-10).
7. If processing with the PRP2 protocol, the message “Close PRP line. Open PPP line. Then press
Play button” is displayed in the message area. Perform the actions as prompted. This allows air to
enter the bowl during emptying.
The fill clamp closes, the Empty clamp opens, and the pump rotates clockwise to move the remaining
blood components from the bowl to the RBC bag.
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