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HAPSITE ER Operating Manual
25 If a compound was not correctly identified, type in the correct name.
Alternately, the down arrow next to the compound name can be used to select
a different name if AMDIS has identified more than one possible match. (See
Figure 11-25.)
Figure 11-25 Correcting the Identification
26 Set the IS Ref. (IS Reference). When using internal standards, best practice is
to use a quant ion from the internal standard that is close in mass to the quant
ion of the compound to be quantified. The software always selects the largest
mass fragment in the spectrum as the quant ion. To change the quant ion,
highlight the field and type in the new number. (See Figure 11-26.)
NOTE: The software will automatically recognize TRIS and BPFB. It will
automatically enter the concentration from the IS canister into the
method for calibration and quantization.
Figure 11-26 IS Reference
27 More than one quant ion can be used from a single internal standard peak. For
example, highlight the second internal standard and right-click. Select
Duplicate Row. Then, change the name of the internal standard peaks to
BPFB_79 and BPFB_117. The Quant Ion should be changed to 79 and 117.
(See Figure 11-27.)
Figure 11-27 Adding Quant Ions
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