4.3 Washing and Cleaning
HA-8190V 4-47
Installing the column
1 Remove the cell washing set.
a Wrap the cell washing set with tissue paper and turn the push
screw on the IN side by hand to disconnect it from the cell
washing set.
b Turn the push screw on the OUT side by hand to disconnect it
from the cell washing set.
c Press the tube until it is protruding about 3 mm from the push
screws on both the IN and OUT sides.
2 Install the column.
a Remove the sealing screws from both ends of the column.
b Check again that the tube protrudes about 3 mm from the push
screw, fit the push screw over the IN side of the column, and then
tighten the push screw lightly by hand.
• Do not fully tighten the push screw at this point.
c Wrap the connection between the IN side of the column and push screw with tissue paper.
• The tissue paper blots up any liquid that overflows during priming.
b
OUT-side tube
a
IN-side tube
NOTE:
Liquid may leak or an abnormal chromatogram may
result after installing the column unless the tubes
protrude about 3 mm from the push screws.
REFERENCE:
Keep the sealing screws in the accessory case.
OUT side
IN side
b
c
IMPORTANT:
Install the column with the arrow to the top. Do not install
the column upside-down.
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