SYSMEX XP Series - Manual Procedures

Sysmex XP-300 9-3
Quality Control
Revised October 2012
Procedure
Preparing control blood
(1) Remove a vial of control material from the refrigerator and
equilibrate to room temperature (18-30ºC) for 15 minutes before
use.
(2) Place the vial between the palms and roll it back and forth 10
times (see illustration).
(3) Turn the vial upside down and roll 10 more times.
(4) Repeat steps (2) and (3) 8 times or for a total of 2 minutes.
Examine the bottom of the vial and assure thoroughly mixing
by confirming that there is no pellet of cells adhering to the
bottom of the vial before performing the analysis. If there still is
pellet of cells, repeat step (3).
(5) Analyze the control blood sample in the same manner as patient
whole blood, according to procedures listed in the instrument’s
Instructions for Use. Wipe threads of cap and vial with clean
lint-free tissue before replacing cap. Recap vial tightly.
(6) Store at 2-8ºC in an upright position.
Manual procedures
Reference method can be applied to EIGHTCHECK-3WP-N,
EIGHTCHECK-3WP-L and EIGHTCHECK-3WP-H. Refer to a
manual for clinical laboratory procedures.
Methodology
Principle of the method
EIGHTCHECK-3WP-N, EIGHTCHECK-3WP-L and
EIGHTCHECK-3WP-H are to be used as hematology control bloods
for the quality control of any Sysmex fully automated and semi-
automated hematology analyzers. EIGHTCHECK-3WP-N is for the
normal level, EIGHTCHECK-3WP-L is for the low abnormal level
and EIGHTCHECK-3WP-H is for the high abnormal level.
The WBC histogram of EIGHTCHECK-3WP-N, EIGHTCHECK-
3WP-L and EIGHTCHECK-3WP-H indicate 3 part distributions
when the instrument’s particle distribution analysis is used.
The use of stabilized cell preparation for controlling hematology
instrumentation is an established procedure. When handled like a
patient sample measured in QC mode and assayed on a properly
calibrated (HGB, HCT) and functioning instrument,
EIGHTCHECK-3WP-N, EIGHTCHECK-3WP-L and
EIGHTCHECK-3WP-H will provide values within the expected
range indicated on the assay sheet.
Note:
Always check with current control materials insert
instructions.