7-8 Sysmex XP-300
Sample Analysis
January 2012
7.8 Analysis in whole blood (WB) mode
Sample preparation
In this mode, the instrument can be used to analyze whole blood to
which an EDTA anticoagulant has been added.
• The blood sample should be collected by vein-puncture.
• Required sample volume: 1 mL or more of whole blood (for a
13 mm diameter sample tube)
500 μL or more of whole blood (for
a micro tube)
• Aspirated sample volume: Approx. 50 μL
Blood in which clotting or platelet aggregation has
occurred cannot be analyzed.
• Lysing of red blood cells or platelet aggregation may
occur depending on the anticoagulant, and correct
analysis results may not be obtained. For the
anticoagulant, use either of EDTA-2K, EDTA-3K, or
EDTA-2Na.
• Mix the sample promptly after the blood is drawn. If
mixing is insufficient, platelet aggregation may occur.
• When analyzing a refrigerated sample, take it out of
the refrigerator at least 30 minutes prior to analysis,
to bring it back to room temperature. Once restored
to room temperature, mix the blood sufficiently
before performing analysis.
• Depending on the sample, separation of the WBC
into 3 categories may become less accurate with the
passage of time after the blood is drawn.
• The sample should be analyzed within 4 hours after
collection. If it is not possible to analyze the sample
within 4 hours, store it in a refrigerator at 2 to 8°C
until it can be analyzed.
• Use sample tubes that are 80 mm or less in height.
All performance claims given in this manual were
generated using specimens in EDTA anticoagulant. Results
may be affected by the use of other anticoagulants.
Therefore, each laboratory should develop protocols for
handling specimens collected in their anticoagulants.
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