Sysmex XP-300 10-3
Calibration
January 2012
10.2 Samples used for calibration
For calibration, use 5 or more samples of fresh normal blood meeting
the following conditions:
• Blood from healthy person who is not taking any medication
• Blood added with an appropriate amount of anticoagulant
• Per-sample whole blood volume to exceed 2 mL
• HGB value to exceed 10.0 g/dL
• HCT value to be within 35.5% and 55.5%
10.3 Establishing reference values
5 or more normal blood samples prepared for calibration of HGB and
HCT should be accurately analyzed 3 times each in accordance with
the following reference methods. The measurements thus obtained
are used as reference values.
HGB values: Cyanmethemoglobin method
HCT values: Microhematocrit method
(5 samples are used for automatic calibration.)
For calibration, do not use EIGHTCHECK-3WP.
EIGHTCHECK-3WP was not prepared for calibration
but for the use as control blood.