SYSMEX XP Series - Analysis in Pre-Diluted (PD) Mode

Sysmex XP-300 7-17
Sample Analysis
Revised July 2012
7.9 Analysis in pre-diluted (PD) mode
Sample preparation
In this mode, the instrument can be used to analyze samples which
have been diluted at the ratio of 1:26.
• The blood sample should be collected by skin puncture of the
earlobe, fingertip, or from a babies’ heel.
• Required blood volume (whole blood): Approx. 20 μL
or more
• Required sample volume (pre-diluted blood): 500 μL or more
(for the micro
tube)
• Aspirated sample volume (pre-diluted blood): Approx. 200 μL
Information
• Platelet aggregation tends to occur on micro samples
collected from earlobes or fingertips. To prevent
aggregation, the sample should be diluted and
analyzed immediately after collection. If the analysis
is performed more than 30 minutes after the sample
is diluted, correct analysis results may not be
obtained.
• When a sample tube containing a general
anticoagulant is used, lysing of red blood cells or
platelet aggregation may occur depending on the
anticoagulant, and correct analysis results may not
be obtained. For the anticoagulant, use either of
EDTA-2K, EDTA-3K, or EDTA-2Na.
Note:
All performance claims given in this manual were
generated using specimens in EDTA anticoagulant. Results
may be affected by the use of other anticoagulants.
Therefore, each laboratory should develop protocols for
handling specimens collected in their anticoagulants.