SYSMEX XP Series - Histogram Error Flags

Sysmex XP-300 8-21
Display and Output of Analysis Results
Revised July 2012
8.3 Histogram error flags
Various information can be obtained from the histograms. The
XP-300 extracts the characteristics of the histogram and displays
them as histogram flags. (For details about histogram error flags, see
“Analysis of histogram” in “14. Technical Information”.)
When the histogram flags are displayed, perform analysis again. If
afterwards the flags are still displayed, the sample is considered to
correspond to one of the following.
Flag Probable sample cause Correction (reference)
[WL] Incomplete lysing of red blood cells, presence of
nucleated red blood cells, increase of large
platelets, platelet aggregation or agglutination,
precipitation of fibrin, etc.
1) Centrifuge sample and replace the plasma with
equal volume of saline or CELLPACK and
repeat analysis.
2) Check smear, etc.
[RL] Presence of fragmented red blood cells, increase
of large platelets, platelet aggregation or
agglutination, etc.
1) Manual red blood cell count of sample
2) Check smear, etc.
[PL] Effects of cryoglobulins, fragmented red blood
cells, or cellular fragments of white blood cells,
etc.
1) Warm sample at 37°C for 30 minutes and
repeat analysis.
2) Check smear, etc.
[WU] Incomplete lysing of red blood cells, presence of
immature white blood cells, white blood cell
aggregation, platelet satellite phenomenon, etc.
1) Centrifuge sample and replace the plasma with
equal volume of saline or CELLPACK and
repeat analysis.
2) Check smear, etc.
[RU] Effects of cold agglutinin, inclusion of white
blood cells, etc.
1) Warm sample at 37°C for 30 minutes and
repeat analysis.
2) Check smear, etc.
[PU] Increase of large platelets, inclusion of
fragmented red blood cells, precipitation of
cryoglobulins, etc.
1) Manual platelet count of sample
2) Check smear, etc.
[DW]
(RBC)
Significant anisocytosis, etc. 1) Check smear, etc.
[DW]
(PLT)
Inclusion of fragmented red blood cells, non-
uniformity in size of platelets, effects of
cryoglobulins.
1) Check smear, etc.
2) Centrifuge sample and replace the plasma with
equal volume of saline or CELLPACK and
repeat analysis, warm sample at 37°C for
30 minutes and repeat analysis, etc.
[MP]
(RBC)
Effects of anemia treatment or blood transfusion
causing the presence of cells of multiple sizes.
1) Check smear, etc.
[MP]
(PLT)
Platelet aggregation, sample with low values for
platelets.
1) Check smear, etc.
[T1] Presence of CML or other immature granulocytes,
incomplete lysing of red blood cells, etc.
1) Check smear, etc.
2) Centrifuge sample and replace the plasma with
equal volume of saline or CELLPACK and
repeat analysis, warm sample at 37°C for
30 minutes and repeat analysis, etc.