14-30 Sysmex XP-300
Technical Information
Revised October 2012
Analysis of histogram
Analysis of histogram allows use of the flagging system that suggests sample error or instrument
error.
Histograms of WBC, RBC, and PLT can be calculated respectively within the ranges given below.
WBC: Approx. 30 - 300 fL (particle after lyse dripping)
RBC: Approx. 25 - 250 fL
PLT: Approx. 2 - 30 fL
Analysis of WBC histogram
1. WBC Histogram
The WBC histogram is discriminated into small, middle, and large WBC by 3-part differential
method using 4 discriminators. The LOWER discriminator (LD) is automatically determined at an
optimum position between 30 and 60 fL. The UPPER discriminator (UD) is fixed at 300 fL, which is
used as the monitor for histogram error. The WBC histogram troughs in the (LD)-to-(UD) range are
determined; the 1st one is defined TROUGH Discriminator 1 (T1) and the 2nd one TROUGH
Discriminator 2 (T2).
• WBC Histogram
1) LYM# [W-SCC (WBC-Small Cell Count)]
Lymphocytes between discriminator (LD) and (T1), which is considered highly correlated with
lymphocyte count.
2) MXD# [W-MCC (WBC-Middle Cell Count)]
Mixed cells between discriminator (T1) and (T2), which is considered highly correlated with the
sum of monocytes, basophils, and eosinophils.
3) NEUT# [W-LCC (WBC-Large Cell Count)]
Neutrophils more than discriminator (T2), which is considered highly correlated with
neutrophils.
4) LYM% [W-SCR (WBC-Small Cell Ratio)]
Ratio of lymphocytes to whole WBC
5) MXD% [W-MCR (WBC-Middle Cell Ratio)]
Ratio of mixed cells to whole WBC
300
WBC
UD
200100
LD T1 T2
[fL]
Lymphocytes Mixed cells Neutrophils
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