5.5 If This Happens
HA-8190V 5-29
Replacing the column IN tube (lower tube)
1 Remove the old tube.
a Turn the push screw on the injection valve by hand to remove the
tube from the valve.
b Turn the push screw at the bottom of the column by hand to
remove the tube from the column.
2 Prepare a spare tube.
a Insert both ends of a spare tube into the new push screws.
• Press the tube until it protrudes about 3 mm from the push screw.
3 Connect the spare tube to the injection valve.
a Wrap one end of the spare tube with tissue paper.
• The tissue paper blots up any liquid that overflows during fluid
pumping.
b Connect the push screw on the other end of the spare tube to the
injection valve.
• Insert the tube into the valve until the tube stops, and then tighten
the push screw lightly by hand.
• Do not fully tighten the push screw at this point.
Column
IN tube
b
a
NOTE:
The column IN tube is 9.5 cm long. Prepare a spare tube with the proper length.
3 mm
3 mm
9.5-cm spare tube
NOTE:
Liquid may leak or an abnormal chromatogram may result
after replacing the tube unless both ends of the tube
protrude about 3 mm from the push screws.